Journal: Gene

Article Title: Renal LRP2 expression in man and chicken is estrogen-responsive

doi: 10.1016/j.gene.2012.07.041

Figure Lengend Snippet: Localization of LRP2 in chicken kidney. A: Immunofluorescence staining (see ) of paraffin sections of female kidney with anti-chicken LRP2 antiserum (1:100) or pre-immune serum (1:100), and nuclear staining with DAPI (magnification, 40 ×). B: Paraffin sections of laying hen (B1. and B3.) and rooster (B2.) kidneys were incubated with anti-chicken LRP2 antiserum (1:1000) or with preimmune serum (1:1000). After the primary antibody, biotinylated goat α-rabbit secondary antibody (1:500 dilution) was used, and slides were incubated with Streptavidin Peroxidase Polymer (1:200). AEC + Substrate-Chromogen was used to detect LRP2 by color reaction. Magnification, 20 ×.

Article Snippet: For biotin staining, the sections were rinsed 3 × 5 min in PBS and incubated with biotinylated goat α-rabbit secondary antibody (1:500 dilution, Sigma) for 1 h. After rinsing 3 × 5 min in PBS, the slides were incubated with Streptavidin Peroxidase Polymer (1:200, Sigma) for 1 h. For the color reaction, the sections were incubated with AEC + Substrate-Chromogen (ready-to-use solution, DAKO).

Techniques: Immunofluorescence, Staining, Incubation, Polymer